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Use of Hydrocyclones for Mammalian Cell Retention

2006-9-6  As the overflow is commonly used for product harvest and cells are discarded, the application of the hydrocyclone has no detrimental effect on the reactor perfusion system. The results indicate that only cells passing from the primary vortex downwards into the inner secondary vortex and from there upwards could be damaged.

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Separation of CHO cells using hydrocyclones.

2007-11-14  The cell number balances (comparison of the amount of cells leaving the hydrocyclone through the underflow and overflow streams with the amount of cells fed to the equipment) closed satisfactorily, with an average deviation of ±9.9%, which is approximately the same deviation associated to the cell counting technique in Neubauer chambers used

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Application of an Inclined Settler for Cell Culture-Based

of cell retention devices that potentially enable continuous virus harvesting are: acoustic settler (Petiot et al.,2011), centrifuge, hydrocyclone (Elsayed et al.,2006), and inclined settler (Woodside et al.,1998;Castilho,2014). However, few studies applying these technologies for

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A 3D-printed mini-hydrocyclone for high throughput

A 3D-printed mini-hydrocyclone for high throughput particle separation: application to primary harvesting of microalgae. The separation of micro-sized particles in a continuous flow is crucial part of many industrial processes, from biopharmaceutical manufacturing to water treatment.

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Separation of CHO cells using hydrocyclones

2007-11-14  Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell

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9.5.1.9: Hydrocyclone Separation of Targeted Biochemical

2016-5-10  Goals. Evaluate hydrocyclone separation to harvest algal lipids. Employing turbulent mixing with concomitant heat transfer Nanostructured adsorbent materials based on magnetic separations and/or flotation. Couple hydrocyclone with nanostructured adsorbents to yield efficient separation process Technology spans between biomass processes.

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Hydrocyclone an overview ScienceDirect Topics

Application of hydrocyclone for algae harvesting was studied by Mohn (1980). It was reported that only Coelastrum algae that grow in large aggregates are harvested by this method. The solid concentration of harvested algae slurry was low, with incomplete solid-liquid separation.

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Harvesting of microalgae by centrifugation for

The most prevalent centrifugal devices that have been identified to be effectively useful to harvest algal biomass include disc-stack centrifuge, solid-bowl centrifuge, hydrocyclone, tubular centrifuge, and multi-chamber centrifuge.

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Hydrocyclone Separation of Targeted Algal Intermediates

2015-4-17  Hydrocyclone technology appears to be a viable alterative to existing algal dewatering efforts that can bring down algal harvesting costs Hydrocylones are expected to be employed at large-scale outdoor algal facilities to replace settling, dissolved air flotation, and centrifuge unit operations Hydrocyclone gains are realized

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Hydrocyclone Separation of Targeted Algal

2015-4-17  Hydrocyclone technology appears to be a viable alterative to existing algal dewatering efforts that can bring down algal harvesting costs Hydrocylones are expected to be employed at large-scale outdoor algal facilities to replace settling, dissolved air flotation, and centrifuge unit operations Hydrocyclone gains are realized

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9.5.1.9: Hydrocyclone Separation of Targeted

2016-5-10  1.1 Hydrocyclone Separation Approach Hydrocyclone unit Classification of components in fluid mixture density and/or size Continuous-flow dewatering Replaces: centrifugation, filtration, and washing Hydrocyclone structure Cylindrical-conical body

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Separation of CHO cells using hydrocyclones

2007-11-14  High cell separation efficiencies, varying from 97.9% to 99.9%, were obtained for 5 out of 6 hydrocyclone configurations. The geometry 3010 (D u = 3.0 mm and D o = 1.0 mm) presented a flow ratio of 100%, diverting the total feed flow to the underflow, indicating that this configuration is inappropriate to perform any kind of cell

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Separation of CHO cells using hydrocyclones.

2007-11-14  The cell number balances (comparison of the amount of cells leaving the hydrocyclone through the underflow and overflow streams with the amount of cells fed to the equipment) closed satisfactorily, with an average deviation of ±9.9%, which is approximately the same deviation associated to the cell counting technique in Neubauer chambers used

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Filtration and separation technology: What’s new with

2009-5-22  The centrifuge, including inertial sedimentation devices such as the hydrocyclone, is a separating device for solids from liquids or for liquids from liquids. Cell harvesting — getting cultural . Peter Rose of Alfa Laval takes a look at one of the growth areas of biopharmaceutical filtration and separation: the harvesting of mammalian

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separation: Application to primary harvesting of

2017-6-2  Brief comparison has been made between the conventional techniques for primary harvesting of microalgae based on the concentration factor and power consumption in the table S3. Technique Concentration factor Power consumption (kWh/m3) Continuous Ref Macro-hydrocyclone 4 0.3 Yes (1) Decanter bowl centrifuge 11 8 No (2) Vacuum filter 2-18.5 Yes

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Frontiers Application of an Inclined Settler for Cell

2020-7-2  Examples of cell retention devices that potentially enable continuous virus harvesting are: acoustic settler (Petiot et al., 2011), centrifuge, hydrocyclone (Elsayed et al., 2006), and inclined settler (Woodside et al., 1998; Castilho, 2014). However, few studies applying these technologies for virus vaccine production were reported.

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Application of an Inclined Settler for Cell Culture-Based

of cell retention devices that potentially enable continuous virus harvesting are: acoustic settler (Petiot et al.,2011), centrifuge, hydrocyclone (Elsayed et al.,2006), and inclined settler (Woodside et al.,1998;Castilho,2014). However, few studies applying these technologies for

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Acoustic Settler, Centrifuge, Hydrocyclone Animal

2021-6-22  Hence higher acceleration provokes higher damage. The cell damage can be significantly reduced by using the centrifuge in intermittent mode, i.e. several minutes per hour or so (Johnson et al. 1996). Hydrocyclone. A detailed description of the hydrocyclone for mammalian cell

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Very High Density of CHO Cells in Perfusion by ATF or

Refine Technology), perfusion floating filter,4 hydrocyclone,9 CellTank.12 This study focussed on TFF and ATF as these technologies were the only ones among those named above, reported to support high cell density, having moderate equip-ment cost, allowing renewal of the device during the culture,

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9.5.1.9: Hydrocyclone Separation of Targeted

2016-5-10  1.1 Hydrocyclone Separation Approach Hydrocyclone unit Classification of components in fluid mixture density and/or size Continuous-flow dewatering Replaces: centrifugation, filtration, and washing Hydrocyclone structure Cylindrical-conical body

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separation: Application to primary harvesting of

2017-6-2  Brief comparison has been made between the conventional techniques for primary harvesting of microalgae based on the concentration factor and power consumption in the table S3. Technique Concentration factor Power consumption (kWh/m3) Continuous Ref Macro-hydrocyclone 4 0.3 Yes (1) Decanter bowl centrifuge 11 8 No (2) Vacuum filter 2-18.5 Yes

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Frontiers Importance of Downstream Processing of

2021-1-19  Hydrocyclone is a low capital cost centrifuge; Cell harvesting techniques should focus on strategies to avoid the use of centrifugation and filtration. The use of flocculants should be extensively studied in H. pluvialis as well as its influence on the molecule purification. On the other hand, the flotation technique has shown very

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Hydrocyclone for mAb production in a perfusion single

2020-10-20  For the first time, a hydrocyclone specially designed for mammalian cell separation is reported to enable perfusion processes at cell densities in the range of 20 to 50 million/mL for 20 to 25 days. Also for the first time, a HC is connected to a single-use bioreactor.

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Hydrocyclone Procedure for Starch-Protein Separation in

2019-11-2  The hydrocyclone system (Fig. 1) consisted of a 10-mm hydro-cyclone (Doxie 5) connected to a positive displacement pump (Hydra Cell, Wanner Engineering, Minneapolis, MN) fed by an overhead tank. The Doxie 5 unit consisted of six 10-mm hydro-cyclones in parallel operation. Five of these hydrocyclones were

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Frontiers Application of an Inclined Settler for Cell

2020-7-2  Examples of cell retention devices that potentially enable continuous virus harvesting are: acoustic settler (Petiot et al., 2011), centrifuge, hydrocyclone (Elsayed et al., 2006), and inclined settler (Woodside et al., 1998; Castilho, 2014). However, few studies applying these technologies for virus vaccine production were reported.

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Products and Solutions for the Biopharmaceutical Industry

2018-7-16  Hydrocyclone 104 BioPAT Cell Harvesting Seed Bioreactor Single-Use Bioreactors 62 Sterile Filtration Sterile Liquid Filters 172 Virus Filtration Virus Clearance 506 Crossflow Buffer Exchange Crossflow Consumables 456 Crossflow Holders & Systems 472

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「production line hydrocyclone specific energy

Hydrocyclone technology is a better alternative to existing algal dewatering technologies because it can bring down algal harvesting costs as it has a relatively lowenergy requirement, particlesorting device (0.3 kW h m 3) compared to other available centrifuge methods (Grima et al., 2003). Read More

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Acoustic Settler, Centrifuge, Hydrocyclone Animal

2021-6-22  Hence higher acceleration provokes higher damage. The cell damage can be significantly reduced by using the centrifuge in intermittent mode, i.e. several minutes per hour or so (Johnson et al. 1996). Hydrocyclone. A detailed description of the hydrocyclone for mammalian cell

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Very High Density of CHO Cells in Perfusion by ATF or

Refine Technology), perfusion floating filter,4 hydrocyclone,9 CellTank.12 This study focussed on TFF and ATF as these technologies were the only ones among those named above, reported to support high cell density, having moderate equip-ment cost, allowing renewal of the device during the culture,

get price